Rapid detection ofmecAandspaby the loop-mediated isothermal amplification (LAMP) method
نویسندگان
چکیده
منابع مشابه
Loop-Mediated Isothermal Amplification (LAMP) Method for Rapid Detection of Trypanosoma brucei rhodesiense
Loop-mediated isothermal amplification (LAMP) of DNA is a novel technique that rapidly amplifies target DNA under isothermal conditions. In the present study, a LAMP test was designed from the serum resistance-associated (SRA) gene of Trypanosoma brucei rhodesiense, the cause of the acute form of African sleeping sickness, and used to detect parasite DNA from processed and heat-treated infected...
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An in-house loop-mediated isothermal amplification (LAMP) reaction was established and evaluated for sensitivity and specificity in detecting the presence of Salmonella Typhi (S. Typhi) isolates from Kelantan, Malaysia. Three sets of primers consisting of two outer and 4 inner were designed based on locus STBHUCCB_38510 of chaperone PapD of S. Typhi genes. The reaction was optimised using genom...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
متن کاملLoop-Mediated Isothermal Amplification (LAMP) for the Rapid Diagnosis of Herpes Simplex Virus Type 1 (HSV-1)
Background and Aims: considering difficulties in usual laboratory methods in detection of viral infections, improved DNA-based diagnostic techniques are more reliable. Loop mediated isothermal amplification method (LAMP) is a nucleic acid amplification method that amplifies DNA using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. In this study, the...
متن کاملDetermination of Herpetic Keratitis Frequency Using Loop Mediated Isothermal Amplification (LAMP) Method in Isfahan, Iran
Herpetic keratitis which is resulted from herpes simplex virus type I, is considered the most common cause of corneal blindness that needs being diagnosed and treated in order to prevent irreversible eye effects. The best method to diagnose it is molecular method which has high sensitivity and exclusivity. The current study was done on 25 eye samples collected from patients referred to the Feyz...
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ژورنال
عنوان ژورنال: Letters in Applied Microbiology
سال: 2010
ISSN: 0266-8254,1472-765X
DOI: 10.1111/j.1472-765x.2010.02806.x